[HTML][HTML] In vivo assembly of coatomer, the COP-I coat precursor
M Lowe, TE Kreis - Journal of Biological Chemistry, 1996 - ASBMB
Coatomer, a seven-subunit hetero-oligomeric complex, is the major component of the COP-I
membrane coat of transport vesicles of the early secretory pathway. We have followed the
assembly of this complex in vivo by pulse-chase experiments and immunoprecipitation of
native coatomer subunits and found that it is an ordered process that takes 1-2 h to
complete. During assembly, direct interactions between α-, β′-and δ-COP, β-and δ-COP,
and γ-, ζ-, and δ-COP occur. Coatomer, once it has assembled, is stable with a half-life of∼ …
membrane coat of transport vesicles of the early secretory pathway. We have followed the
assembly of this complex in vivo by pulse-chase experiments and immunoprecipitation of
native coatomer subunits and found that it is an ordered process that takes 1-2 h to
complete. During assembly, direct interactions between α-, β′-and δ-COP, β-and δ-COP,
and γ-, ζ-, and δ-COP occur. Coatomer, once it has assembled, is stable with a half-life of∼ …