Organoids are a reliable method for modeling organ tissue due to their self-organizing
properties and retention of function and architecture after propagation from primary tissue or
stem cells. This method of organoid generation forgoes single-cell differentiation through
multiple passages and instead uses differential centrifugation to isolate mammary epithelial
organoids from mechanically and enzymatically dissociated tissues. This protocol provides a
streamlined technique for rapidly producing small and large epithelial organoids from both …