Quantitative and qualitative comparisons of Cryptosporidium faecal purification procedures for the isolation of oocysts suitable for proteomic analysis
Q Truong, BC Ferrari - International journal for parasitology, 2006 - Elsevier
With the recent publication of the Cryptosporidium genome, investigation of the proteins
expressed by Cryptosporidium parvum will provide complementary information on the
biology of this complex organism. Proteomic studies on this apicomplexan parasite have
been hampered due to the inability to culture or isolate high numbers required for 2D gel
analysis. Neonatal calves are a common source of Cryptosporidium oocysts and we report
on the development of a sucrose-Percoll™ purification procedure which produced the high …
expressed by Cryptosporidium parvum will provide complementary information on the
biology of this complex organism. Proteomic studies on this apicomplexan parasite have
been hampered due to the inability to culture or isolate high numbers required for 2D gel
analysis. Neonatal calves are a common source of Cryptosporidium oocysts and we report
on the development of a sucrose-Percoll™ purification procedure which produced the high …