Crystal structure analysis of human Sirt2 and its ADP-ribose complex
S Moniot, M Schutkowski, C Steegborn - Journal of structural biology, 2013 - Elsevier
Sirtuins are NAD+-dependent protein deacetylases that regulate metabolism and aging-
related processes. Sirt2 is the only cytoplasmic isoform among the seven mamalian Sirtuins
(Sirt1-7) and structural information concerning this isoform is limited. We crystallized Sirt2 in
complex with a product analog, ADP-ribose, and solved this first crystal structure of a Sirt2
ligand complex at 2.3 Å resolution. Additionally, we re-refined the structure of the Sirt2
apoform and analyzed the conformational changes associated with ligand binding to derive …
related processes. Sirt2 is the only cytoplasmic isoform among the seven mamalian Sirtuins
(Sirt1-7) and structural information concerning this isoform is limited. We crystallized Sirt2 in
complex with a product analog, ADP-ribose, and solved this first crystal structure of a Sirt2
ligand complex at 2.3 Å resolution. Additionally, we re-refined the structure of the Sirt2
apoform and analyzed the conformational changes associated with ligand binding to derive …