Herpes simplex virus type 1 propagation, titration and single-step growth curves
L Grosche, K Döhner, A Düthorn, A Hickford-Martinez… - Bio …, 2019 - bio-protocol.org
Given the endemic seroprevalence of herpes simplex viruses (HSV), its associated human
diseases, and the emergence of acyclovir-resistant strains, there is a continuous need for
better antiviral therapies. Towards this aim, identifying mechanistic details of how HSV-1
manipulates infected cells, how it modulates the immune responses, and how it causes
diseases are essential. Measuring titers and growth kinetics of clinical isolates and viral
mutants are important for a thorough characterization of viral phenotypes in vitro and in vivo …
diseases, and the emergence of acyclovir-resistant strains, there is a continuous need for
better antiviral therapies. Towards this aim, identifying mechanistic details of how HSV-1
manipulates infected cells, how it modulates the immune responses, and how it causes
diseases are essential. Measuring titers and growth kinetics of clinical isolates and viral
mutants are important for a thorough characterization of viral phenotypes in vitro and in vivo …