An imaging protocol to discriminate specialized conduction tissue during congenital heart surgery
We performed preclinical validation of intraoperative fiber-optic confocal microscopy (FCM)
and assessed its safety and efficacy in an ovine model of the pediatric heart. Intraoperative
imaging was performed using an FCM system (Cellvizio, Mauna Kea Technology, Paris,
France) with specialized imaging miniprobe (GastroFlex UHD, Mauna Kea Technologies).
Before imaging, we applied an extracellular fluorophore, sodium fluorescein, to fluorescently
label extracellular space. We imaged arrested hearts of ovine (1–6 months) under …
and assessed its safety and efficacy in an ovine model of the pediatric heart. Intraoperative
imaging was performed using an FCM system (Cellvizio, Mauna Kea Technology, Paris,
France) with specialized imaging miniprobe (GastroFlex UHD, Mauna Kea Technologies).
Before imaging, we applied an extracellular fluorophore, sodium fluorescein, to fluorescently
label extracellular space. We imaged arrested hearts of ovine (1–6 months) under …