Generating monoclonal antibodies from single B cells is a valuable tool for characterizing
the specificity and functional properties of humoral responses. We and others developed
protocols that have facilitated major advances in our understanding of B cell development,
tolerance, and effector responses to HIV and influenza. Here, we demonstrate various
refinements and dramatically reduce the time required to produce recombinant antibodies.
Further, we present new methods for cloning and isolating antibodies from cells with lower …