[HTML][HTML] Zinc potentiates an uncoupled anion conductance associated with the dopamine transporter
Binding of Zn 2+ to an endogenous binding site in the dopamine transporter (DAT) leads to
inhibition of dopamine (DA) uptake and enhancement of carrier-mediated substrate efflux.
To elucidate the molecular mechanism for this dual effect, we expressed the DAT and
selected mutants in Xenopus laevis oocytes and applied the two-electrode voltage clamp
technique together with substrate flux studies employing radiolabeled tracers. Under voltage
clamp conditions we found that Zn 2+(10 μm) enhanced the current induced by both DA and …
inhibition of dopamine (DA) uptake and enhancement of carrier-mediated substrate efflux.
To elucidate the molecular mechanism for this dual effect, we expressed the DAT and
selected mutants in Xenopus laevis oocytes and applied the two-electrode voltage clamp
technique together with substrate flux studies employing radiolabeled tracers. Under voltage
clamp conditions we found that Zn 2+(10 μm) enhanced the current induced by both DA and …