Use of RNA polymerase II to transcribe artificial microRNAs
Y Zeng, X Cai, BR Cullen - Methods in enzymology, 2005 - Elsevier
MicroRNAs (miRNAs) are endogenously encoded∼ 22-nt-long RNAs that are generally
expressed in a highly tissue-or developmental-stage-specific fashion and that
posttranscriptionally regulate target genes. Regulatable RNA polymerase II promoters can
be used to overexpress authentic microRNAs in cell culture. Furthermore, one can also
design and express artificial microRNAs based on the features of existing microRNA genes,
such as the gene encoding the human miR-30 microRNA. Overexpression or inappropriate …
expressed in a highly tissue-or developmental-stage-specific fashion and that
posttranscriptionally regulate target genes. Regulatable RNA polymerase II promoters can
be used to overexpress authentic microRNAs in cell culture. Furthermore, one can also
design and express artificial microRNAs based on the features of existing microRNA genes,
such as the gene encoding the human miR-30 microRNA. Overexpression or inappropriate …