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A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid

K Burton - Biochemical journal, 1956 - ncbi.nlm.nih.gov
K Burton
Biochemical journal, 1956•ncbi.nlm.nih.gov
MATERIALS AND METHODS Deoxyribonucleic acid (DNA). Two samples of highmolecular-
weight calf-thymus DNA were used. Sample I was kindly given by Dr FW Putnam and had
been pre-pared according to Hammarsten (1924). Sample II was kindly given by Dr PW Kent
and had been prepared according to Kay, Simmons & Dounce (1952). In addition, a
commercial sample of lower molecular weight (British Drug Houses Ltd.) was also used.
DNA was isolated from bacteriophage T2r which had been obtained by confluent lysis as …
MATERIALS AND METHODS Deoxyribonucleic acid (DNA). Two samples of highmolecular-weight calf-thymus DNA were used. Sample I was kindly given by Dr F. W. Putnam and had been pre-pared according to Hammarsten (1924). Sample II was kindly given by Dr P. W. Kent and had been prepared according to Kay, Simmons & Dounce (1952). In addition, a commercial sample of lower molecular weight (British
Drug Houses Ltd.) was also used. DNA was isolated from bacteriophage T2r which had been obtained by confluent lysis as previously described, and purified by three cycles of differential centrifuging (Burton, 1955). A portion (20 ml.) of a preparation con-taining 1011 infective bacteriophage/ml. was shaken with 5 ml. of CHC13 for 1 hr. and centrifuged, and the clear supernatant poured into 2 vol. of ethanol. After 2 hr. at-100, the fibrous DNA was removed by a glass rod and
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