Comparison of glycoprotein components, tryptophan, lipid peroxidation and antioxidants in borderline and severe hypertension and myocardial infarction
KN Srinivasan, KV Pugalendi, G Sambandam… - Clinica chimica acta, 1998 - Elsevier
KN Srinivasan, KV Pugalendi, G Sambandam, MR Rao, S Krishnan, VP Menon
Clinica chimica acta, 1998•Elsevier2.2. Methods Venous blood was collected in heparinised tubes from normal and
hypertensive subjects after an overnight fast and within 3 h of the onset in myocardial infarct
(ie, before the initiation of treatment). The plasma was separated by centrifugation at 1300 g
for 15 min and stored at 4 C until analysis. The buffy coat was removed and the erythrocytes
were washed three times with physiological saline. Aliquots of erythrocytes were kept at 4 C
until analysis. The erythrocyte membrane was prepared by the method of Dodge et al.[16]. A …
hypertensive subjects after an overnight fast and within 3 h of the onset in myocardial infarct
(ie, before the initiation of treatment). The plasma was separated by centrifugation at 1300 g
for 15 min and stored at 4 C until analysis. The buffy coat was removed and the erythrocytes
were washed three times with physiological saline. Aliquots of erythrocytes were kept at 4 C
until analysis. The erythrocyte membrane was prepared by the method of Dodge et al.[16]. A …
2.2. Methods
Venous blood was collected in heparinised tubes from normal and hypertensive subjects after an overnight fast and within 3 h of the onset in myocardial infarct (ie, before the initiation of treatment). The plasma was separated by centrifugation at 1300 g for 15 min and stored at 4 C until analysis. The buffy coat was removed and the erythrocytes were washed three times with physiological saline. Aliquots of erythrocytes were kept at 4 C until analysis. The erythrocyte membrane was prepared by the method of Dodge et al.[16]. A lipid extract was prepared from erythrocyte membranes according to the procedure of Folch et al.[17].
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