Pancreatic acinar cells submitted to stress activate TNF-α gene expression
MI Vaccaro, A Ropolo, D Grasso, EL Calvo… - Biochemical and …, 2000 - Elsevier
MI Vaccaro, A Ropolo, D Grasso, EL Calvo, M Ferreria, JL Iovanna, G Lanosa
Biochemical and Biophysical Research Communications, 2000•ElsevierTo elucidate whether pancreatic acinar cell submitted to stress is able to express TNF-α, we
studied TNF-α mRNA expression by Northern blot and in situ hybridization in healthy
pancreas, in tissue from caerulein-induced pancreatitis and after lipopolysaccharide (LPS)
treatment. In specimens from normal pancreas, TNF-α mRNA expression, as judged by both
Northern blot and in situ hybridization, was negative, whereas a strong but transient
expression was observed in acinar cells from caerulein pancreatitis and LPS treatment. TNF …
studied TNF-α mRNA expression by Northern blot and in situ hybridization in healthy
pancreas, in tissue from caerulein-induced pancreatitis and after lipopolysaccharide (LPS)
treatment. In specimens from normal pancreas, TNF-α mRNA expression, as judged by both
Northern blot and in situ hybridization, was negative, whereas a strong but transient
expression was observed in acinar cells from caerulein pancreatitis and LPS treatment. TNF …
To elucidate whether pancreatic acinar cell submitted to stress is able to express TNF-α, we studied TNF-α mRNA expression by Northern blot and in situ hybridization in healthy pancreas, in tissue from caerulein-induced pancreatitis and after lipopolysaccharide (LPS) treatment. In specimens from normal pancreas, TNF-α mRNA expression, as judged by both Northern blot and in situ hybridization, was negative, whereas a strong but transient expression was observed in acinar cells from caerulein pancreatitis and LPS treatment. TNF-α mRNA appeared as rapidly as 30 min after treatment, and was maximal 6 h after. At this time, there was mild infiltration consisting mostly of polymorphonuclear leukocytes (PMNL) and no signal of TNF-α transcript was found in their cytoplasm. Our results strongly indicate that pancreatic acinar cell is the source of TNF-α early in the course of acute pancreatitis and LPS treatment, and suggest that the expression of this cytokine is a part of a general response of the acinar cell to aggression.
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