Transformation of chicken cells by the gene encoding the catalytic subunit of PI 3-kinase
Science, 1997•science.org
The avian sarcoma virus 16 (ASV 16) is a retrovirus that induces hemangiosarcomas in
chickens. Analysis of the ASV 16 genome revealed that it encodes an oncogene that is
derived from the cellular gene for the catalytic subunit of phosphoinositide 3-kinase (PI 3-
kinase). The gene is referred to as v-p3k, and like its cellular counterpart c-p3k, it is a potent
transforming gene in cultured chicken embryo fibroblasts (CEFs). The products of the viral
and cellular p3k genes have PI 3-kinase activity. CEFs transformed with either gene showed …
chickens. Analysis of the ASV 16 genome revealed that it encodes an oncogene that is
derived from the cellular gene for the catalytic subunit of phosphoinositide 3-kinase (PI 3-
kinase). The gene is referred to as v-p3k, and like its cellular counterpart c-p3k, it is a potent
transforming gene in cultured chicken embryo fibroblasts (CEFs). The products of the viral
and cellular p3k genes have PI 3-kinase activity. CEFs transformed with either gene showed …
The avian sarcoma virus 16 (ASV 16) is a retrovirus that induces hemangiosarcomas in chickens. Analysis of the ASV 16 genome revealed that it encodes an oncogene that is derived from the cellular gene for the catalytic subunit of phosphoinositide 3-kinase (PI 3-kinase). The gene is referred to as v-p3k, and like its cellular counterpart c-p3k, it is a potent transforming gene in cultured chicken embryo fibroblasts (CEFs). The products of the viral and cellular p3k genes have PI 3-kinase activity. CEFs transformed with either gene showed elevated levels of phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate and activation of Akt kinase.
AAAS